FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005093104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274447333
Sequences flagged as poor quality0
Sequence length75
%GC31

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA7537842127.465532339496264TruSeq Adapter, Index 12 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA58507612.131833797051327TruSeq Adapter, Index 12 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC17427260.6349946931348027TruSeq Adapter, Index 12 (100% over 62bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA16121240.5874074207163091TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15981450.582313911572972TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15350060.5593080403517713TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15091790.5498974916254697TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15057470.5486469784714578TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA14762950.5379155934446629TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA14590220.5316218540188911TruSeq Adapter, Index 12 (98% over 62bp)
ATTGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA14493290.5280900288435304TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA14436460.5260193218929914TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCATACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA13602030.49561530991448915TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGTACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA13081050.47663243278811535TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACA5607330.20431351759574212TruSeq Adapter, Index 12 (100% over 62bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)