FastQCFastQC Report
Mon 4 Jul 2022
EGAF00005093110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280002940
Sequences flagged as poor quality0
Sequence length75
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA7856791328.059674301991254TruSeq Adapter, Index 12 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA62095282.217665285943069TruSeq Adapter, Index 12 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC17546370.6266494916089095TruSeq Adapter, Index 12 (100% over 62bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA16777400.5991865656839175TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA16602750.5929491311769798TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15901810.5679158225981484TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15759980.5628505186409828TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15708080.5609969666747071TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15376100.5491406625944714TruSeq Adapter, Index 12 (98% over 62bp)
ATTGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15275620.5455521288455043TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15167450.5416889551231141TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA14990840.5353815213511687TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCATACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA14177680.5063403977115384TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGTACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA13689240.4888962951603294TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACA5121590.18291200799534463TruSeq Adapter, Index 12 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA3372570.12044766387095793TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA3158980.11281952968065265TruSeq Adapter, Index 12 (98% over 62bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)