FastQCFastQC Report
Mon 4 Jul 2022
EGAF00005093112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268111227
Sequences flagged as poor quality0
Sequence length75
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA7833938129.21898567119683TruSeq Adapter, Index 12 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA59620922.2237382845590425TruSeq Adapter, Index 12 (100% over 63bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA16755660.6249518227000617TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA16574290.6181870929261758TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15788330.5888723936204283TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15687840.5851243223022511TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15641310.5833888485393415TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15529690.5792256510019255TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15281030.5699511419564687TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA14944060.5573828506629452TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC14817590.5526657785203453TruSeq Adapter, Index 12 (100% over 62bp)
ATTGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA14778600.5512115313246468TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCATACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA14126970.5269070660737382TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGTACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA13524330.5044298275506381TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACA4949190.18459465705253736TruSeq Adapter, Index 12 (100% over 62bp)
ACCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA3876510.1445858886021211TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA2749880.1025648955759693TruSeq Adapter, Index 12 (98% over 62bp)
GCTCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA2709180.10104686888028004TruSeq Adapter, Index 12 (98% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)