FastQCFastQC Report
Fri 27 May 2022
EGAF00005093180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363223483
Sequences flagged as poor quality0
Sequence length75
%GC22

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA73847342.033110287640736TruSeq Adapter, Index 12 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA30458360.838557015874439TruSeq Adapter, Index 12 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC8023730.22090339351764876TruSeq Adapter, Index 12 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA7467520.20559023162056955TruSeq Adapter, Index 12 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA3664020.1008750857664123TruSeq Adapter, Index 12 (98% over 62bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)