FastQCFastQC Report
Fri 27 May 2022
EGAF00005093196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385243635
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4598305911.936098308282238TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA9998740.25954328875543914TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA9353240.2427876582568327TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA9321180.241955457615802TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA8781090.2279360176839781TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA8157280.21174340751924425TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC8106990.21043799983872544TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA8081260.20977011080273916TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA8021760.2082256336305206TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7898820.20503440634392311TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7802340.20253001714097105TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7768650.20165550561270143TruSeq Adapter, Index 9 (98% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7681000.19938032201362652TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5471120.14201714195745246TruSeq Adapter, Index 9 (98% over 63bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4125330.10708366408182189TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4042000.10492061731272989TruSeq Adapter, Index 9 (98% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)