FastQCFastQC Report
Fri 27 May 2022
EGAF00005093198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382132200
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4549453611.905444241547821TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA9912920.2594107484268533TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA9262880.2423998815069759TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA9205580.24090040043733557TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA8768760.2294692779095821TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC8181460.2141002511696214TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA8101510.21200804328973064TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7978650.20879292558962578TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7942780.20785424520624016TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7816620.204552769957622TruSeq Adapter, Index 9 (98% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7726170.2021857880597343TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7723860.202125337775775TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7674660.20083782523430374TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA6197840.16219099044780838TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA4531130.11857493296822408TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3913330.10240775312836761TruSeq Adapter, Index 9 (98% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)