FastQCFastQC Report
Fri 27 May 2022
EGAF00005093202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363517660
Sequences flagged as poor quality0
Sequence length75
%GC21

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA15154990.41689831520152276TruSeq Adapter, Index 2 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA9360060.2574857023452451TruSeq Adapter, Index 2 (100% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)