FastQCFastQC Report
Sun 15 May 2022
EGAF00005093269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395456677
Sequences flagged as poor quality0
Sequence length75
%GC24

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAGGGGGGGG20533750.5192414541024427Illumina Single End PCR Primer 1 (100% over 56bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGGGG4985720.12607499860218568Illumina Single End PCR Primer 1 (100% over 56bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)