FastQCFastQC Report
Fri 27 May 2022
EGAF00005093274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393257024
Sequences flagged as poor quality0
Sequence length75
%GC24

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA55351881.407524255688819TruSeq Adapter, Index 6 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC10171540.2586486541687301TruSeq Adapter, Index 6 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA9751610.24797039607358673TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA5313120.13510553342335216TruSeq Adapter, Index 6 (98% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3973740.10104689191768891TruSeq Adapter, Index 6 (100% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)