FastQCFastQC Report
Fri 27 May 2022
EGAF00005093276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392330006
Sequences flagged as poor quality0
Sequence length75
%GC24

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA46536581.1861590826167907TruSeq Adapter, Index 6 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA13316650.3394247137956611TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC9769080.24900160198299998TruSeq Adapter, Index 6 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA5120850.13052404663639214TruSeq Adapter, Index 6 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC4576670.11665358065933913TruSeq Adapter, Index 6 (98% over 62bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)