FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005093327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14828801
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGGTACGCTACTGACCCACATTTAACGTTTCCCAACGCACTTTTAACA156150.10530183795709443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTA40500.057.933676
CGCTACT64050.037.192888
GCTACTG67700.035.1883629
ACGCTAC77850.030.4148247
GTACGCT90650.026.8358845
AGGTACG97700.024.6051643
AAGGTAC124400.019.5551832
GGTACGC130500.018.8620974
AAAGGTA181400.013.8463121
CTAGTTA7350.013.71291885-89
GATCTAG7800.012.36788180-84
CTACTGA40400.012.14964510-14
TAACTAG6600.010.034003135-139
TCTACTA45900.010.0080781
ATCTAGT9200.09.85988685-89
CTATTCT54100.09.8446785
TAACGTT53950.09.63395225-29
ACTATTC55850.09.1497564
TCTAGTT11850.08.38397485-89
ACACACT61250.08.134006115-119
Produced by FastQC (version 0.11.5)