FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005093334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23079889
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC1166290.505327386973135No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG687110.297709404061692No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA419870.18192028566515203No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA265520.11504388084362105No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTGGTAATCTCGTAT263120.11400401449071094TruSeq Adapter, Index 10 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA74300.016.7612937
ACACTAT79850.016.047058
GAGCACA278600.015.47732459
AGAGCAC289900.014.7747098
GATCGGA306100.014.0839111
CACTATA93000.013.7006299
TCGGAAG382350.011.3967283
ATCGGAA412550.010.8597382
AAGAGCA408000.010.7979537
GAAGAGC471350.09.5146096
CAAACAC161050.08.6716835
AAACACT150950.08.6316126
GGAAGAG526400.08.588285
ACCAAAC157700.08.5091383
CGGAAGA555350.08.1302594
CCAAACA176000.07.5694214
TACCAAC150400.06.7967765
AGTCACT130600.06.7356325-29
TACCAAA226900.06.64532332
CTCGTAT143100.06.358603540-44
Produced by FastQC (version 0.11.5)