FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005093351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18836147
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATAG68500.028.7897019
GTGAGTA53000.026.4879172
AGTGAGT53600.026.106921
TAACCCC59200.022.3703779
GCCCTTA78850.020.2682974
ACACTAT101700.020.102697
AAACACT114250.018.0204435
CACTATA113900.017.4438428
TAGCCCT94500.017.2935092
TAGCATG64900.016.1944038
CAAACAC128450.015.8045084
AACACTA135900.015.2555376
ATAACCC96400.014.2630748
GGACGTC86600.013.8821068
TACAAAC153150.013.7263452
GTCCCTT89850.013.5964171
GTCTCTT84150.012.5817361
CCCTTAT133850.012.2623045
CTTGGAC99800.012.1180945
TTGGACG101700.012.104026
Produced by FastQC (version 0.11.5)