FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005093358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17824563
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA67650.019.4710589
GCCACTC82100.016.7454368
AGCCACT124150.010.8417937
AAGCCAC128950.010.3824626
CGTTAAA78700.09.8779095
CCGTTAA83950.08.9172034
ACAAAGC183850.07.96252443
TACAAAG199400.07.4862372
GTTAAAT104100.07.46761136
ATACAAA229850.07.41000181
TCCGTTA117550.07.1776393
GAGCACA140900.06.33456759
AAAGCCA252450.05.87364675
ACACACT57850.05.300631550-54
AGAGCAC161600.05.25589758
CTACACA59050.05.24167350-54
CCCTAAT59200.05.229016100-104
Produced by FastQC (version 0.11.5)