FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005093359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17824563
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA67550.022.055059
GCCACTC84100.017.9754688
AGCCACT122550.012.218197
AAGCCAC127450.011.8048616
CGTTAAA76850.011.0533155
CCGTTAA81500.010.95290854
ACAAAGC183800.08.8517343
GTTAAAT104900.08.7153066
TACAAAG199950.08.3891542
TCCGTTA120050.07.9754443
ATACAAA240400.07.4146161
AAAGCCA256400.06.4855745
ACACACT59050.05.87569750-54
GTTCCGT184500.05.69243531
TAGCCAC67450.05.378942525-29
CCCTAAT62500.05.298188100-104
CCTAATG64550.05.1743693105-109
TACACAC69750.05.118818350-54
CTACACA66450.05.004713550-54
Produced by FastQC (version 0.11.5)