FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005093360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19932404
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC404290.20283052661384948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTCAT81800.011.3517646
TCTCTCA85800.010.9066055
GCTCTCT115250.08.3138413
CTTAGTC31750.07.70784048
CTCATTA80750.07.2200478
CTCTCTC138050.06.7786074
CCTTAGT38601.2732926E-115.9674617
TTAGTCC37704.0017767E-115.9185859
GGGGGGG73850.05.65807252
TCTCATT123100.05.14578447
TGCTCTC207550.05.13724142
Produced by FastQC (version 0.11.5)