FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005093372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15202637
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG266670.17541035808458758No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA170230.11197399503783455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCC56450.014.025169
GTGAGTA57200.013.9768852
AGTGAGT60450.013.5938851
ATAACCC87900.09.1708218
GAGTATA105050.08.1534234
GTATAAC106350.08.1213036
TGAGTAT120100.06.7755273
AGAGCAC205800.06.43504438
GAGCACA200550.06.4240589
TATAACC143550.06.2673787
GATCGGA241400.05.48271371
CTAACTA35800.05.1877007125-129
Produced by FastQC (version 0.11.5)