FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005093390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17693215
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGAC17400.012.4112886
TTAGACC17800.012.1324527
TAGTTAG18650.010.8110764
CTTGGGA69850.08.4616491
CTTGCGA117000.07.98289731
AGTTAGA27450.07.86753135
TAGACCC31700.07.49379878
CTAGAAC11350.00164285337.0718531
CTTCCCA96500.06.57852171
CTTGGCA145150.06.38444381
CTTGGGT39150.06.3369981
CTTGCTA42800.06.30803871
CTTGGGG89200.06.2988651
GTAGTTA37901.8189894E-126.2819963
CTTGGGC125450.06.2237121
CTTCCGA155250.05.7810921
CTTGTGA76450.05.72677561
CTTGGCC215100.05.46162461
CTTGGCG289300.05.4480581
GTTGGGA79850.05.3915481
Produced by FastQC (version 0.11.5)