FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005093399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18527275
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA210300.014.11963751
AGAGCGT242950.011.8829328
TCGGAAG260950.011.3116263
ATCGGAA290550.010.4070322
GAGCGTC284050.010.1368169
AAGAGCG296900.09.8934137
GAAGAGC313550.09.3447936
GGAAGAG330150.08.8313245
CGGAAGA386950.07.8146684
GTGCACT82800.07.3209630-34
TAGATCT85150.07.304855340-44
TAGGGAA92800.06.82583215-19
GTAGGGA91750.06.605822615-19
TGTAGGG99700.06.13683815-19
TCATTAA106200.06.08677865-69
AGAGTGT106950.05.909433425-29
AGTGTGC105700.05.897596425-29
CGTATCA115900.05.66447960-64
GTAGATC117550.05.658898440-44
TGTGCAC112050.05.56406730-34
Produced by FastQC (version 0.11.5)