FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005093478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14413210
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC166050.11520681374933134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA177250.09.5428659
AGAGCAC198850.08.3614878
GATCGGA243150.07.3403461
TCGGAAG252250.06.9974133
AAGAGCA263200.06.8372967
ATCGGAA273550.06.5052332
GGAAGAG334850.05.50329735
CTAGATC14150.05.292258140-144
GAAGAGC372300.05.0076856
Produced by FastQC (version 0.11.5)