FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005093479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14413210
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG144150.10001241916269867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG246050.07.0229083
GATCGGA243100.06.97781231
AGAGCGT256100.06.8034218
TGTGTAG51100.06.537722640-44
ATCGGAA274950.06.4195392
GTGTGTA55150.06.187718435-39
AAGAGCG295400.06.0445127
GGAAGAG331150.05.58765945
CTAGATC16200.05.3322845140-144
GAGCGTC325100.05.3153579
TACAGTG67600.05.239166730-34
TAGATCT66550.05.21468940-44
GAAGAGC369950.05.00161366
Produced by FastQC (version 0.11.5)