FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005093595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14778071
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT39250.015.2530131
TAACCCC45700.013.0722939
GTGAGTA47250.012.4935882
CCTTAGT58200.011.0075157
CTTAGTC59050.010.9702618
AGCACAC67250.09.2044129
CTCTCTC66650.09.1813634
TTAGTCC76000.08.902349
TCCTTAG79500.08.601596
GCTCTCT80600.08.3062583
CTACCTC95400.07.59255551
ATAACCC90300.07.1737978
CTCCTTA110000.07.0672975
TCTCTCT84550.06.81080775
TACCTCC116650.06.04806572
CTCTCTT94450.05.86828236
CAGCACA124800.05.7097228
TGCTCTC109250.05.66698652
ACCTCCT144200.05.29173563
GAGTATA140500.05.12403544
Produced by FastQC (version 0.11.5)