FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005093871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16091209
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTTA39300.031.3197864
CCCTTAG39250.030.8095155
CTTAGAG54500.023.1138177
TTAGAGC57100.021.430898
TAGAGCC64300.019.1432329
CCTTAGA66500.018.9422556
TGCCCCT114900.010.9008242
GCCCCTT118800.010.724413
CACAAAC105700.06.8779916
CTGCCCC227250.06.13093851
TAGGTCG51700.05.95936990-94
CGCACAA116650.05.61528644
CCCCCCA57550.05.103110310-14
Produced by FastQC (version 0.11.5)