FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005093931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18417596
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG1037180.563146243407663No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA731130.39697363325810814No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA540480.29345849480029856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA418650.06.64693451
TTCCCTA87250.06.2701138
GAGCGTC442500.06.24662079
ATCGGAA471100.06.2351062
TCGGAAG452200.06.2246463
AGAGCGT436200.06.2048238
TCCCTAC76000.05.87226069
AAGAGCG516500.05.61654147
CGGAAGA532600.05.48773774
TCCTCCT403550.05.03635071
Produced by FastQC (version 0.11.5)