FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005093998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16579054
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGTA47400.016.40722
AGTGAGT48250.015.4683591
TAACCCC55100.013.5882039
CCACTCA57850.011.2000379
ATAACCC85650.09.3299618
GTATAAC88550.08.8618326
GAGTATA101650.08.003074
TATAACC102650.07.99521547
ACACTAT63250.07.8536738
TGAGTAT109850.07.53769163
GCCACTC87100.07.35624368
AGCCACT97550.06.78960947
GGCCTAA20050.06.751773475-79
AACACTA76700.06.57032637
CACTATA72700.06.43664079
ACCAAAC88450.06.43087153
AAGCCAC116400.06.0612016
AGTATAA145400.05.99084665
TACAAAG124250.05.7955232
AAACACT89700.05.778646
Produced by FastQC (version 0.11.5)