FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005093999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005093999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16579054
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGTA43550.016.2019982
AGTGAGT46750.014.7992981
TAACCCC51750.014.1884629
TACGCTA34600.013.3154936
CCACTCA57500.011.39259
GTATAAC87250.08.9106976
ATAACCC86700.08.7179778
GAGTATA96600.08.1232584
ACGCTAC59700.08.0787847
TATAACC104000.07.8907277
GCCACTC87850.07.86638078
CGCTACT59900.07.57110458
ACACTAT64400.07.37740378
AGCCACT103150.07.0485157
GGCCTAA21550.06.884110575-79
TGAGTAT107800.06.8785883
CACTATA72250.06.87474739
AACACTA74950.06.81918057
GTACGCT74300.06.7820755
AGGTACG75550.06.5749963
Produced by FastQC (version 0.11.5)