FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005094000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005094000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15349514
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCACTAATCTCGTAT261320.17024643255806016TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC256800.023.1291318
GAGCACA274200.021.898589
GATCGGA279300.021.6258161
TCGGAAG289050.020.7475453
ATCGGAA323800.018.8609582
AAGAGCA348700.017.467027
GAAGAGC358550.017.1680536
GGAAGAG362700.016.7324355
CGGAAGA371900.016.2217254
AGTCACT105100.08.35779530-34
AGTCACA116500.08.13255925-29
GTCACTA111350.08.00493835-39
CCAGTCA146900.07.517974425-29
CTCGTAT112500.07.474223640-44
GATTGCA163250.07.3644865
ACTAATC126200.07.23414935-39
AGATTGC166950.07.20127254
TCACAGT129700.07.183381630-34
CACTAAT130900.07.029408535-39
CACAGTC130250.07.020379530-34
Produced by FastQC (version 0.11.5)