FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005094022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005094022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13151024
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA126150.09.0743019
AGAGCAC147350.08.061688
GGCGACC138200.07.4495729
TACTCGT60800.07.27623461
TGATACG146950.06.7609783
TACGGCG148900.06.5757096
ATGATAC167350.06.36941432
ACGGCGA180950.06.28622967
GATCGGA200950.05.81056361
ATACGGC171600.05.7057155
TACATCT109850.05.67778351
TACATAT126900.05.65788361
TACATAG68150.05.5337291
TACATCA154400.05.44867751
AAGAGCA230350.05.438177
CGGCGAC185700.05.3888328
GAAGAGC237900.05.3564526
TACGTCG56650.05.24885231
TACATCG113650.05.2326881
TACTTTC214550.05.20562171
Produced by FastQC (version 0.11.5)