FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005094055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005094055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14317903
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCAAACACTATAGCTCTCAAAATTTAAAGTACTAAATCCTTGAGGCC176450.12323732043721766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTAT77800.035.8061188
CAAACAC88050.031.8813635
AACACTA90400.030.9748047
GCCACTC47850.030.6883898
CCACTCA55800.026.1872129
CACTATA109850.025.6879
CCAAACA118250.023.8608844
AAACACT119350.023.7615856
ACCAAAC124700.022.626943
AAGCCAC77050.019.244046
CCTCTTA103500.016.96884
AGCCACT95200.015.5760627
CGTTAAA62750.013.305955
GTACCTC141150.012.860461
TATTTAG138100.012.6138939
GCCCTAA52000.011.51761545-49
ACAAAGC140250.011.3934773
CCGTTAA71750.011.3359574
GGCCCTA53300.011.2907245-49
CCCCTCA60000.010.31846885-89
Produced by FastQC (version 0.11.5)