FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005094068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005094068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18642903
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC489140.2623733009821485No Hit
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG302900.16247469613503862No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA193830.10396985920057622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT42500.032.523131
GTACGTA44800.030.3814162
GATCTAA34700.029.878863
CACGTAA48750.028.6522083
TACGTAA51200.027.8436553
TAGGCTA36950.024.5572832
TCACGTA60900.022.9408052
GTAGAGT53250.017.0772551
TAGAGTA52850.016.7603972
AGATCTA63300.015.9275342
GTTCCTA64250.015.2436872
AGTGTAA85150.014.5436963
ATCACGT97650.014.45297051
ACAGTGT87300.014.2498241
CAGTGTA85150.014.2085212
ACGTAAT65700.014.1354214
AAGTTAA149400.014.0225455
GTTAAAG155450.013.9855917
AACACTA59950.013.5691897
AGTTAAA164250.013.3245536
Produced by FastQC (version 0.11.5)