FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005094069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005094069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18642903
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG516750.27718322623896074No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA345310.1852232991825361No Hit
AAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA280500.15045940001940683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT44850.029.1408621
GATCTAA35050.028.3451463
GTACGTA47450.027.7725332
TAGGCTA35600.026.2962632
CACGTAA51100.025.6411883
TACGTAA54000.024.664123
TCACGTA61900.021.5219552
ACGTAAT62900.017.624934
TAGAGTA54500.016.7806152
AGATCTA62900.016.3714082
GTAGAGT56100.016.0086271
AGTGTAA82150.014.5474943
AACACTA62950.014.4099497
AGGCTAA66200.014.24626353
ACACTAT65750.013.9057878
AAGTTAA142750.013.7672935
ATCACGT97000.013.6997131
GTTCCTA65400.013.6535192
CACTATA70000.013.26729
GTTAAAG150100.012.9020647
Produced by FastQC (version 0.11.5)