FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005094071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005094071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15282845
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGATACCACCAGTGTAGATCTCGGT162790.10651812538830303Illumina Single End PCR Primer 1 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACA78750.030.9883635
ACACAAG98800.024.9183167
TACACAA107800.023.038276
GGTACAC114100.021.5158274
CACAAGG122400.020.5250878
AGGTACA139750.018.0819243
AAGGTAC160350.015.8496852
ACAAGGC194000.013.3951159
GATCGGA257850.011.8134131
GAGCGTC262300.011.08725559
TCGGAAG308500.010.1746773
AGAGCGT302400.09.8074578
AAGAGCG349450.08.9197447
ATCGGAA367550.08.6384742
AAAGGTA308250.08.321581
CGGAAGA412100.07.8089784
CTAACAC67950.07.71337855-59
GAAGAGC438700.07.46609076
ACACACT80950.07.4351955-59
ACACTGT83100.07.2077966125-129
Produced by FastQC (version 0.11.5)