FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005094080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005094080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17418454
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGAC16950.013.760679115-119
ACGTTAA43450.013.0895454
TACGTTA51750.012.1259923
CTAGACC20900.011.022623120-124
ATCCTAG22050.010.7085285115-119
ACAACAC125850.010.0298213
TCCTAGA24800.09.927479115-119
CACCATA124550.09.7100537
GTTACGT66350.09.4671641
ACCATAC124100.09.4552268
ATACAAC137700.09.3886181
CGTTAAA72200.08.3758595
CTAACAC31900.07.944077525-29
ACACCAT162500.07.93025066
AACACCA161400.07.8504845
CCATACC170450.07.7710019
TACAACA163950.07.5230352
CCTAAGG35450.07.391991645-49
GGTCCTA35500.07.259930640-44
GTTAAAT95550.07.23315576
Produced by FastQC (version 0.11.5)