FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005094114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005094114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14379629
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGATC221900.08.565842
GGTTGAT140750.07.5898711
GGTGGAT289000.07.49348641
TGGATCA252350.07.3325173
CACGAGG132150.06.5919348
GGTTCAC136350.06.44904951
TGGATAG130500.06.23393877
TATTTGG303300.06.1957153
GGATAGC106300.06.16315038
GGATCAC380600.06.1480574
GGTAACA69300.06.08219341
GGTTGTT149850.06.0135431
TCACGAG142650.06.00578557
GGTTTCA503100.05.8356951
GGTCTAG23152.4034283E-55.65050171
GTTCACG101700.05.59280162
GCGCGGT104850.05.5621142
ACGAGGT149050.05.55467849
ATATTTG385500.05.49093442
GCGGATC141650.05.4894712
Produced by FastQC (version 0.11.5)