FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005094136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005094136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16591825
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC197790.11920930940387811No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCATAC97250.017.5446689
ATACAAC94800.017.4014051
CACCATA102300.016.5378368
CCTAGAC21250.014.907579120-124
ACAACCA115450.014.7795693
AACCACC115650.014.5039925
TACAACC114350.014.4811032
CAACCAC116800.014.3005424
AACACTA33850.014.0369167
CTAGACC25050.013.278458120-124
CACTATA37900.012.5369299
CTAACAC28500.012.42824625-29
ATCCTAG29900.011.943208115-119
ACACTAT38750.011.8903518
TCCTAGA31350.011.253019115-119
ACGTTAA32950.010.9250144
GTCCTAA34300.010.11691345-49
CCCCTAA34000.09.825509125-129
GGTCCTA34500.09.7243845-49
CACCTAA37000.09.65079975-79
Produced by FastQC (version 0.11.5)