FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005094137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005094137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16591825
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACAAC93000.017.4576321
ACCATAC99550.015.9093259
TACAACC103850.015.6027842
CACCATA105250.014.8425318
ACAACCA112650.014.5081973
AACCACC116900.014.3491925
AACACTA34050.013.7426087
CAACCAC119500.013.4355574
CCTAGAC22700.013.257059120-124
ACACTAT40100.013.1053568
CACTATA39050.012.5359779
CTAGACC25600.012.092755120-124
ATCCTAG27800.011.757745115-119
CTAACAC29000.011.5677925-29
TCCTAGA28950.011.539378115-119
GTCCTAA35200.09.61206345-49
CACCTAA37150.09.34094375-79
GGTCCTA34550.09.084475545-49
CCCTAAT35100.08.943064125-129
TACGTTA35500.08.7208513
Produced by FastQC (version 0.11.5)