FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005094236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005094236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15725235
Sequences flagged as poor quality0
Sequence length150
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGTAG16600.024.2909533
AGTGAGT22550.018.3716241
GTGAGTA28150.015.8601362
TAGAACC37500.010.9427217
CAAGACA51550.09.728361
GCGCCCC291500.09.4095348
GTCCCTT65050.08.6032521
CTCGCTA28400.08.31517190-94
CTAATAT59400.08.023335130-134
TATACTA60650.08.000725125-129
AAGACAT64050.07.98238752
GAGTAGA51550.07.96047454
TTGCTAA59500.07.9372387100-104
AGACATT59050.07.92607263
TCGCTAA31550.07.85009390-94
GCTAAAT60000.07.7751017105-109
GAGCACA231000.07.6666379
GTAGCAA31950.07.5069291
TAGCAAC29350.07.3604912
CCCGGCC427900.07.23581
Produced by FastQC (version 0.11.5)