FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005094237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005094237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15725235
Sequences flagged as poor quality0
Sequence length150
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGTAG17000.022.0203463
AGTGAGT23850.015.3231461
GTGAGTA27050.014.1118552
CAAGACA53400.011.77127551
AGACATT58400.09.8615873
CTCGCTA29300.09.0910690-94
TAGAACC40300.08.7530767
AAGACAT68200.08.6597412
TCGCTAA32150.08.59865990-94
TTGCTAA56300.07.7745957100-104
GTCCCTT62050.07.7744291
GCGCCCC312500.07.76335678
CTAATAT58400.07.618139130-134
TATACTA58100.07.5336823125-129
TTATAGG61900.07.420093115-119
GCTAAAT56850.07.3954434105-109
TAGCAAC30900.07.22567942
TGCTAAA61950.07.158487105-109
GAGTAGA53200.07.0365764
GTAGCAA35350.07.03000831
Produced by FastQC (version 0.11.5)