FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005094284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005094284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14940433
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC235240.15745192927139393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA173100.06.82492831
AGAGCAC179450.06.4997638
CGATCTA27801.2129021E-86.2157965
GAGCACA184050.06.1808369
TCGGAAG202250.05.8028653
ATCGGAA228500.05.2330252
AAGAGCA256650.05.0215497
Produced by FastQC (version 0.11.5)