FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005094338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005094338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15022154
Sequences flagged as poor quality0
Sequence length150
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA155150.015.6834859
AGAGCAC172650.014.1771848
TCGGAAG208750.012.2789683
GATCGGA216400.011.8981611
AAGAGCA219550.011.8044377
ATCGGAA230500.011.4014562
GGAAGAG242650.010.5915925
GAAGAGC286700.09.0395486
CGGAAGA301950.08.5127824
CCTAGAC5150.07.82807120-124
ACCACTA37951.8189894E-126.2600798
ATCCTAG7453.6379788E-126.1844006115-119
CTAGACC6551.7789716E-95.935077120-124
CACACGT96550.05.32383710-14
TCCTAGA8855.2204996E-105.206077115-119
ACGTTAA22456.118748E-45.1314724
Produced by FastQC (version 0.11.5)