FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005094396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005094396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14905933
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC278040.1865297529513919No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA224650.010.9500851
AGAGCAC234500.010.4070558
GAGCACA244150.09.7303459
CGTGCGA45900.09.2535915
TCGGAAG271650.09.0421593
ATCGGAA312650.08.38632
GTGCGAG54650.07.90372946
GAAGAGC362700.07.1255416
AAGAGCA361850.07.0825957
CGCTAGT13502.1869523E-46.9323516
CGGAAGA367150.06.9249924
GCTAGTA16354.6665482E-56.60456947
GGAAGAG393900.06.4514855
TCGTGCG78100.05.90224274
GAGAGAC64050.05.51066972
ATTCGTG84000.05.48817732
TGCGAGA94300.05.34388737
TCGCTAG18300.0083565295.114035
Produced by FastQC (version 0.11.5)