FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005094397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005094397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14905933
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG205840.13809266417607002No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA155760.1044953039839908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA228300.010.0803091
CGTGCGA47050.09.7920925
GAGCGTC235400.09.1742359
AGAGCGT262100.08.2671278
TCGGAAG275400.08.2599383
GTGCGAG54900.07.99857476
AAGAGCG308000.07.66616637
ATCGGAA314750.07.29929832
CGGAAGA377450.06.274684
GAAGAGC377450.06.2556086
TCGTGCG76150.05.86106974
GGAAGAG412750.05.7031635
TAGATCT83100.05.648648340-44
CATTCGT100900.05.48548561
ATTCGTG86450.05.41507632
TGCGAGA95550.05.3491267
Produced by FastQC (version 0.11.5)