FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005094426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005094426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14502905
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT43300.029.111141
GTGAGTA44600.027.4760592
TAACCCC46000.026.2927469
ATAACCC74950.016.7134118
GTATAAC116700.011.4123056
GAGTATA113400.011.363514
TATAACC117250.010.9289817
TGAGTAT119450.010.6767023
GGACCTA18055.999755E-87.57790046
CCTAGGG7750.06.31678285-89
CTAACTA46800.06.060948125-129
AACCCCC44100.06.007511610-14
CCCTAGG7751.4551915E-115.945206685-89
AGTATAA243850.05.75684265
CGTCTAA47750.05.669234845-49
CTAGGGT9851.8189894E-125.408591785-89
ACTACCC52600.05.338221535-39
TGGACTA73900.05.26061878
GTGGACT77350.05.211957
TACCCCC60500.05.09338135-39
Produced by FastQC (version 0.11.5)