FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005094491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005094491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15788642
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA45700.017.1727317
ACACTAT46600.016.2230478
CACTATA48300.015.8010669
AAACACT73250.011.2054786
CAAACAC86850.09.367895
CCAAACA86800.09.2904554
ACCAAAC97650.08.1108433
TATTTAG60300.06.3282889
TACCAAA130950.06.27073242
CCATACT75750.06.0831019
CTAGACC16100.05.992507120-124
ACTATAG29900.05.827345410-14
ACGTTAA27554.1567728E-75.7496284
ATACCAA166350.05.62935541
CACCATA84500.05.45321137
CCTAGAC17250.05.4260645115-119
GCCCTTA65700.05.36993934
CTAACAC17300.05.1606225-29
GGTCCTA18200.05.142897640-44
ATACAAC102950.05.03783941
Produced by FastQC (version 0.11.5)