FastQCFastQC Report
Fri 27 May 2022
EGAF00005096597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005096597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395241759
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA262256376.635340624521409TruSeq Adapter, Index 18 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA239076986.048879566898193TruSeq Adapter, Index 18 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAC4474160.11320058921203212TruSeq Adapter, Index 18 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)