FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005097013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005097013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13215727
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAA76700.010.865216145
AAAAAAA6975350.09.130811145
CTTGAAA1224700.08.135961145
TTGAAAA1193650.07.898346145
TGAAAAA1162500.07.8419404145
CGTGTTA55250.07.74812562
GTTAGCC120150.07.7185385
TGTTAGC124150.07.6449274
GAAAAAA1167850.07.5764284145
TGCTTGA1461100.07.2510905145
CTGCTTG1539350.07.2308564145
GCTTGAA1397250.07.1882806145
GTCTTCT1927350.06.9821186145
CCGTCTT2019650.06.9249573145
CGTCTTC1941250.06.894794145
TTCTGCT1711650.06.8840094145
TCTGCTT1621000.06.732523145
ATATCGT49750.06.7003984145
CTTCTGC1872600.06.4780803145
GCCGTCT2113150.06.4162216145
Produced by FastQC (version 0.11.5)