FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005097025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005097025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24106748
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTAGC271050.012.7491814
GTTAGCC277150.012.3640155
CGTGTTA131500.012.0304392
GTGTTAG217250.010.9483153
CCGTGTT211000.09.1181051
AAAAAAA18044900.09.0169325145
TGTGTTA113850.08.7324932
CTATGTT123450.08.4686171
CATGTTA122500.08.1751132
TTAGCCA471850.08.1680986
TTGAAAA3073050.08.111635145
CTTGAAA3221900.08.071997145
TGAAAAA2939450.07.984808145
GAAAAAA2932150.07.965145145
GACCTCA696550.07.8420996
ATGTTAG129900.07.81541633
CCATGTT571900.07.50254341
TAGCCAG517450.07.2944047
TCGAACT254150.06.9268637
TATGTTG125900.06.9168162
Produced by FastQC (version 0.11.5)