FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005097052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005097052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19737224
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCC42000.013.465892145
GCGTCGG132250.012.500546145
CGTATCG24400.09.212165145
CGCCGTT44950.08.065479145
TGTTAGC169950.06.91068274
GTTAGCC170400.06.7222495
CCGTATT25209.140422E-96.617853145
AGACGGA135300.06.59197951
ACGGAGT98000.06.5838523
CATTAAA3137850.06.516385145
TTAAAAA3206100.06.4296837145
ATTAAAA3135750.06.398196145
TAAAAAA3251950.06.3791647145
TCATTAA3262500.06.294084145
CGTGTTA80200.06.237282
ATCATTA3393450.06.1772685145
GTATCAT3670950.06.0855956145
TATCGTT25053.7881E-76.07857145
TATCATT3545400.06.0720425145
CCGTATC3988800.05.8678784145
Produced by FastQC (version 0.11.5)