FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005097063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005097063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21543929
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACAAA284150.08.389965145
AAAAAAA6108250.07.4926805145
TATACGC11050.0013306837.21399072
TTGAAAA1079000.07.1723537145
CTTGAAA1064950.07.015221145
GAAAAAA1136850.06.781884145
TGAAAAA1079000.06.7626967145
GCCGTCT1785900.06.633955145
TGCTTGA1296400.06.606788145
GCTTGAA1236050.06.5952067145
CGTCTTC1664700.06.54672145
CCGTCTT1691750.06.4891577145
TATGCCG1967150.06.450013145
GTATGCC2101500.06.4410906145
ATATGAT76350.06.40170761
TCTGCTT1525350.06.3942184145
CTGCTTG1437450.06.336573145
TTCTGCT1618000.06.3146973145
TCGTATG2243650.06.294607145
CGTATGC2153400.06.289216145
Produced by FastQC (version 0.11.5)