FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005097077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005097077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13036279
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG802440.615543745266575TruSeq Adapter, Index 27 (97% over 44bp)
CTTGTGGAAAGGACGAAACACCGGTCCGCAGGTATGCTTGCGTGAATTCCA230980.17718246134498963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCG36800.043.40826822
ACCGGTC37850.042.91738520
GTCCGCA38450.041.66252524
CACCGGT39050.041.5985419
GGTCCGC39050.041.0223823
GGACGAA40800.040.9721611
GACGAAA41500.040.33512512
CCGGTCC39900.040.2048621
CTTGCGT40350.039.97921437
CGTGAAT40400.039.7072841
TCCGCAG40600.039.402225
GCTTGCG41550.039.25776736
CGAAACA42500.039.12122714
ACGAAAC42400.039.10751713
ACACCGG42050.039.00528718
TTGCGTG41450.038.9728238
AGGACGA43300.038.8144110
AAGGACG43350.038.562049
TGCGTGA41800.038.4850239
CGCAGGT41450.038.26865427
Produced by FastQC (version 0.11.5)